FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80728093
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACGTCTGGTGTAGATCTC2573670.31880723356118423Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG794750.050.829179
GAGCGTC883900.045.7693028
AGAGCGT936050.043.3510937
ATCGGAA1030950.039.4516031
TCGGAAG1047850.038.990372
AAGAGCG1052550.038.7112586
CGGAAGA1095300.037.2780233
GAAGAGC1624600.025.3826225
CGCCGTA418850.022.90322155-59
TCGCCGT435950.021.88178655-59
GCCGTAT456200.021.04715555-59
GTCGCCG473750.020.35316755-59
TGGTCGC491400.019.87269650-54
CGTATCA496300.019.19176360-64
GTGGTCG523000.018.7135650-54
GGAAGAG2308300.018.1817044
TAACGTC570550.017.0750930-34
TCTCGGT588650.016.902545-49
CCGTATC480700.016.79216455-59
GTAACGT584600.016.54566430-34
Produced by FastQC (version 0.11.5)