FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66767819
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA444950.020.8853421
TCGGAAG458650.020.2348522
CGGAAGA484300.019.222953
TATGCCG158000.013.93956845-49
CTCGTAT177550.012.55211440-44
CGTATGC182600.011.96633245-49
TCTCGTA177400.011.94153740-44
AGAGCAC850650.011.3430797
ATGCCGT198400.011.14491645-49
AGCACAC880000.011.1130459
TATCTCG190550.011.0109140-44
GAGCACA917800.010.5921598
GAAGAGC950900.010.4752215
CGTTCTA209600.010.44603935-39
GCCGTCT214350.010.13270350-54
TCGTATG200950.09.78442540-44
ATCTCGT220300.09.747745540-44
TGCCGTC205500.09.41928845-49
CTCGTTC245650.09.084022530-34
CCTCGTT251100.08.97924930-34
Produced by FastQC (version 0.11.5)