FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54946678
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGACTTGGTGTAGATCTC1397760.25438480557459725Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG488050.044.9543239
GAGCGTC552000.039.9038168
AGAGCGT604800.036.539957
ATCGGAA650400.033.911821
TCGGAAG657800.033.556512
AAGAGCG693800.032.0301786
CGGAAGA691100.031.9979343
GAAGAGC1047850.021.4497665
CGCCGTA221600.021.41158355-59
TCGCCGT239750.019.78459755-59
GCCGTAT250800.018.9533655-59
GTCGCCG266700.017.790855-59
CGTATCA272550.017.4570260-64
TGGTCGC278550.017.37295350-54
GTGGTCG290000.016.71700750-54
CCGTATC272850.016.43904355-59
GGAAGAG1460800.015.6986124
GGTCGCC289750.015.61150-54
GTACGAC308800.015.29840530-34
TACGACT319700.014.949059530-34
Produced by FastQC (version 0.11.5)