FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005101203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24737799
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG156800.016.3198879
GAGCGTC185150.014.0167658
AGAGCGT202100.012.9128487
CGCCGTA41550.012.31733355-59
AAGAGCG233300.011.2790486
ATCGGAA232450.011.1121371
TCGGAAG235700.010.8600492
TCGCCGT48700.010.65778855-59
CGGAAGA245750.010.5307573
GCCGTAT53450.09.90052355-59
GTCGCCG58150.09.299767555-59
TGGTCGC64100.08.57258450-54
CCGTATC61200.08.504642555-59
CGTATCA64000.08.33701860-64
GGTCGCC68400.07.71570950-54
GTGGTCG72950.07.592217450-54
GAAGAGC411000.06.75513465
TCTCGGT93300.06.4959245-49
TCGGTGG95400.06.094555445-49
CGTCGTG143800.05.8991466145
Produced by FastQC (version 0.11.5)