FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005101778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604761
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT5250.071.637372
AGACGTG6600.056.9842721
TGATCGG1000.00741288751.652103145
CGTGTGC7500.050.1461564
ACGTGTG7750.049.427223
CGACCCC2500.00780425427.547789145
GTGCTCT16200.024.0756637
GTGTGCT16350.023.0028255
TGTGCTC17000.022.5329976
TCCGATC4500.019.1917410-14
ACGTTAC1950.00862423317.858329
GCTCTTC21450.017.8583189
CGTTGGA7300.017.1733441
TTCCGAT5250.016.45006410-14
CCGATCT5050.015.44656215-19
CTTCCGA5500.014.6892810-14
TGCTCTT28650.013.6134628
TCTTCCG6300.013.04507710-14
ACGGGAT6304.455045E-612.1606659
AACGGGA7853.5053017E-711.5339728
Produced by FastQC (version 0.11.5)