FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005101779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604761
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT5650.077.685852
ACGACGT300.002267072269.670644
CGTGTGC8450.053.5928044
AGACGTG7600.052.252981
ACGTGTG9350.049.179283
CGACGTG952.527353E-644.002511
GATCGGG2150.003887900431.768883145
GTGCTCT18050.026.6330997
GTGTGCT18750.026.0103745
TGTGCTC18850.025.1331796
GCTCTTC18800.024.8294329
ACGGGTT2508.11771E-519.507789
CGTTGCA1800.005423609619.3529571
TCCGATC5000.019.22909710-14
TGCTCTT26750.017.4502188
AACGGGT2902.5295131E-416.8170538
CGTTGGA8800.015.8342361
TTCCGAT6250.015.38327910-14
CCGATCT5950.015.222157515-19
AACAACG3100.007609805613.4846412
Produced by FastQC (version 0.11.5)