FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429322
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA350.00445757558.742668
GACGTGT4451.0913936E-1121.5609762
ATCTCGT4750.005508375419.3145
ACACGTC6650.001953667316.542858145
CGTGTGC6653.2105163E-914.42802054
AGACGTG7551.9064828E-812.7081241
ACGTGTG9453.6568963E-810.878273
TCCGATC3251.5301175E-610.5435546
CGTTGGA12602.2737368E-1010.3343561
TATCCAC27150.010.0969586
TTCCGAT3404.030244E-710.0783985
AACGGGA12051.2498276E-89.668578
AAACGGG13751.1170414E-88.9716067
ATCCACT31200.08.7862957
CTCGTAT2500.009163228.716649140-144
CCGATCT3951.9995646E-48.6750767
GAAACGG14352.3152097E-88.5964866
CCGAGTA13054.3524415E-78.4025262
CCCGAGG7500.00412815258.2239711
CCACTTG34750.08.085929
Produced by FastQC (version 0.11.5)