FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415983
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT2950.051.471232
AGACGTG4650.032.653791
TATGGCG950.006013945729.0603121
ACGTGTG5600.027.1143073
CGTGTGC5600.027.1143074
TCCGATC2550.012.99166910-14
CCGATCT2350.012.922564515-19
GTGTGCT14200.011.6650565
CGTTGGA6608.2770475E-611.5030391
CTTCCGA3250.011.04291910-14
GTGCTCT13900.010.923757
TATCCAC14201.8189894E-1210.6929676
TTCCGAT3100.010.68669510-14
GCTCTTC15500.010.6866959
AATATCC20450.010.4624234
CGAGTAG7352.7258771E-510.329263
TGTGCTC15307.2759576E-129.9241916
CCGAGTA7152.346097E-49.6529012
CCTAAGT8952.3425058E-48.4826891
CCAAATA27950.08.3957791
Produced by FastQC (version 0.11.5)