FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415983
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3700.061.578812
TGGTCGC1302.7536825E-454.416775145
AGACGTG4400.045.5055581
CGACGTG659.3331665E-442.4879461
GATCGGG1700.001033418941.61283145
CGTGTGC5950.040.6134764
ACGTGTG5900.039.7874373
CGCTAGA850.003484193732.490788
GTGCTCT12250.019.162937
GTGTGCT13050.019.046325
TGTGCTC13850.016.9491626
GCTCTTC16600.014.9731629
TCCGATC3700.013.80858110-14
CCCCAAT8002.7739588E-912.9455461
CCGATCT3750.012.51978115-19
TGCTCTT22700.011.5578448
TTCCGAT4400.011.29793210-14
ACGGGAT4350.00576969411.1103539
CTTCCGA4400.010.984110-14
TCTTCCG5100.09.20572110-14
Produced by FastQC (version 0.11.5)