FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198338
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT4204.2740794E-818.332769
CGTTGGA5401.2369128E-1018.147581
AAACGGG6607.2759576E-1216.9691647
AACGGGA5353.924106E-815.7004448
GAAACGG7801.0004442E-1014.3585246
GGAAACG7559.495125E-1013.906855
CGACAGA5300.00187041110.5657088
CCTCAAA22702.764864E-107.4006491
TATCCAC13403.5503697E-57.31320336
ATCCACT14951.973973E-57.0231927
CCACTTG16256.302061E-56.4613369
CCCAAAT19602.9664825E-66.428371
GTTGGAA15502.385734E-46.3223832
CCCTGGA13550.00235205076.19906761
CCCAAGT16354.7474672E-45.99369571
ATATCCA18152.8705935E-45.78494265
CCCAGAA18253.092941E-45.75324441
CCCAGGT21106.264289E-55.6396341
TCCACTT23605.9029895E-55.33881578
CCAAATT17050.0050580015.3370761
Produced by FastQC (version 0.11.5)