FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198338
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT1203.225721E-426.57855145
CGTTGGA6650.022.1089341
GACGTGT1902.7783803E-422.1089322
ACGGGAT4504.620233E-918.6697659
AACGGGA6403.6379788E-1217.5029058
GGAAACG7950.016.7323365
GAAACGG8301.8189894E-1215.1832446
AAACGGG8351.8189894E-1215.0923257
TCCGATC1452.3394894E-414.4851646
CGTGTGC3700.001546353513.2454414
AGCGTCG1306.3330156E-412.027339140-144
CGTCGTG1306.3330156E-412.027339140-144
CGAACTG1150.00365627819.74676855-59
GTTGGAA17651.0913936E-119.1233282
ATCGGAA3101.5762143E-68.396086135-139
CCAAGAC10258.61763E-47.5134422
CCTAATT10800.00150172667.13081362
TCGGAAG3201.78026E-47.049213135-139
CCCATTA10550.0094321256.63617321
CGGAAGA3953.4172257E-46.150044135-139
Produced by FastQC (version 0.11.5)