FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643518
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG2000.00210976135.988247145
CGTCGTG3155.6313915E-428.562101145
GACGTGT2804.8748916E-1027.0108952
AGACGTG3951.1441443E-920.8875981
ACAATCG1950.009289334517.6294882
CGTGTGC5858.8439265E-915.278894
ACGTGTG6101.5359547E-814.6527053
TCCGATC2358.3255145E-814.6287247
GCGTCGT2800.00821820712.852945145
CCGATCT3000.00889972111.45916758
CGTTGGA8359.1915535E-710.7043721
TTCCGAT3850.0046896818.9292216
ATCGGGA2401.5628058E-48.806721135-139
ATCGGAA6400.08.406415135-139
AAACGGG9857.470965E-58.3762457
GTGCTCT13607.7464574E-78.0888247
GATCGGA5951.0186341E-107.7504525135-139
GGAAACG10701.9554274E-47.71084175
GAAACGG11756.938372E-57.6069376
CCCGGGT8150.0084516287.5925771
Produced by FastQC (version 0.11.5)