FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942135
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGG700.003828170261.036827145
CCGTAAC1050.00876709626.8998092
GACGTGT1801.8180364E-423.5373332
CGTTGGA4956.730261E-1018.5445651
CGAGATT2851.9979916E-417.3432983
AACGGGA4401.2731871E-616.0481828
ACGGGAT3154.285529E-415.6915559
CCCGAAT2850.00398461914.86568451
AGACGTG2900.004472853614.6093791
GGAAACG6151.9410982E-713.7779515
AAACGGG5551.4113688E-512.7228827
GAAACGG6152.913679E-612.6297886
GAGCAGG11950.00437329949.534345145
GTTGGAA11957.1251634E-89.4543272
CCTCATA7750.0042328158.2001031
CCTGAGG16902.6173439E-87.93862721
CCAGAAA18151.1210432E-87.78093621
ATCCACT11855.5262113E-57.7464647
CCCAAGA11202.4326632E-47.5655711
GCCTGGA12308.778661E-57.4630571
Produced by FastQC (version 0.11.5)