FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525470
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC2200.006086687628.986671145
ACGGGAT5650.028.43199
AACGGGA7300.025.832568
CGTTGGA8150.025.7092951
AAACGGG9100.023.0253567
GAAACGG10050.021.543796
GGAAACG10150.021.3315375
GGGGGGG14203.7394693E-612.3499565145
CAAAGCG5652.5999462E-411.1255261
GTTGGAA22350.010.3124752
GAGATTT24752.0833053E-58.373928145
TGGAAAC33150.07.79551274
TGAACGC4256.75517E-57.502433145
CCCATAA12006.0976157E-46.98435831
TTGGAAA43050.06.8140083
CCCAAGT18751.4872021E-66.7049841
GAACGAT3401.1399646E-46.190838385-89
GGCTAAT22758.478364E-76.1400951
CCTTGAT12600.00774398356.0974561
GGCCATT13750.002842596.095441
Produced by FastQC (version 0.11.5)