FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108226
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGG1000.0035257962.321167145
GACGTGT3950.025.8679312
CGTCGTG3656.473262E-422.765722145
CCGATCG650.002384160622.303415140-144
CGATCGG757.151934E-419.219435140-144
AGCGTCG3652.6493293E-417.074291145
GCGTCGT3800.002905669616.400307145
CGTGTGC7055.11136E-1014.4933814
AGACGTG6252.3910616E-814.1687271
ACGTGTG7651.7407729E-913.3566443
GCGGTGT3150.009816444512.9750252
CGGCTGA5451.6964006E-512.4988782
CGTAATT3950.003105828512.0717011
GCGCTCC6951.3254685E-611.7615341
GCGGCTG6508.086581E-611.5278111
TCCCGTT9706.62847E-710.708104145
CGCTCCA7102.151834E-510.553632
TCCGATC2401.1914381E-910.21783310-14
CCCGTTT11204.5780325E-89.7312692
TATCCAC21200.00219001048.819033145
Produced by FastQC (version 0.11.5)