FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669001
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3609.5296855E-920.893672
CCGGGAT3104.8474187E-415.4404842
CGTATAA2750.003892355114.9191171
AGACGTG5155.6837416E-714.6052861
TTCCGAT2705.3473344E-512.6628319
ACGTGTG6003.170886E-612.53620153
CGTGTGC7151.7809543E-611.4762454
CTTCCGA3600.00217193911.3965478
ACGGGAT6206.0943188E-511.0289179
TCCGATC2409.822543E-1110.8267210-14
AAACGGG8952.0829975E-710.6962017
GAAACGG9605.4566954E-79.9719796
CGTTGGA7755.4078293E-59.7054461
AACGGGA7052.2364076E-49.699198
GCAGTGT16250.0082839238.822526145
TATCCAC17209.276846E-118.7461886
GGAAACG10752.4480189E-58.2691235
ATCCACT21251.2732926E-118.0446217
CCCAAGT23702.0008883E-117.5015251
GATCGGA4402.929246E-66.879342130-134
Produced by FastQC (version 0.11.5)