FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669001
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGC750.002241254869.86746145
CGGCGGG3650.001582884623.927216145
GACGTGT3455.7807483E-921.819822
GAGCGCC3700.00386188318.883099145
TCCGATC3101.0913936E-1117.6606167
CGTCGTG3551.9540717E-514.760733145
CCGATCT3751.4586747E-514.59944258
TTCCGAT3852.1871569E-514.2202366
AGCGTCG4005.486947E-613.100149145
CTTCCGA4253.5499298E-412.8818625
GTCGTGT4101.3025492E-412.780635145
CGTGTGC6003.1421623E-612.5463964
AGACGTG5402.0641892E-411.4058151
ACGTGTG6701.0753247E-511.23557953
AGCGGCG2151.0932194E-511.220033140-144
CGTTGGA7856.168107E-59.5896021
TAGATCG1903.2565353E-49.343935130-134
TCGGAAG8000.08.731057135-139
AAACGGG8701.8902773E-48.6526877
AACGGGA7200.00289126748.5543618
Produced by FastQC (version 0.11.5)