FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064586
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3550.074.004842
AGACGTG4600.054.1065141
CGTGTGC5800.045.2960624
ACGTGTG5850.044.908923
GTGTGCT11450.022.3409235
GTGCTCT12200.021.5341957
GCTCTTC12850.021.5209669
TGTGCTC13400.020.12176
GCGTCGT1700.008853978519.943907145
TCCGATC3700.017.56430610-14
TTCCGAT3700.017.56430610-14
AAAGGTC7353.4446895E-416.145067145
TGCTCTT19400.013.8984958
TCTTCCG4600.013.22603710-14
CTTCCGA4550.013.06748310-14
CCGATCT4100.012.81547215-19
AACGGGA4707.597948E-411.7678478
AAACGGG6356.94301E-510.8875747
CGTTGGA5853.93944E-410.6363231
GGAAACG7151.9715348E-510.6363235
Produced by FastQC (version 0.11.5)