FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181034
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG300.002285132969.532235
GACGTGT5250.058.2746242
CGATCGA400.007139675352.1491663
AGACGTG6850.044.6630331
CGTGTGC8950.034.183444
ACGTGTG9750.031.3786453
GCCGAGC4100.00376180520.614908145
GTGTGCT20900.015.9691235
GTGCTCT20050.015.6057377
TCCGATC4950.014.88972910-14
TGTGCTC23150.013.8163396
GCGCTTG3600.001325275313.5201555
GCTCTTC24850.012.8711579
CGTTGGA10558.731149E-1111.86331751
TTCCGAT5900.011.31371810-14
CCGATCT5650.010.82979815-19
GCGCGGT5200.001688217710.6972652
AAACGGG13203.6379788E-1110.5351857
CTTCCGA6150.010.1754475
CGCGGTG5600.0031244729.9331753
Produced by FastQC (version 0.11.5)