FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181034
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT7050.072.011872
CGACGTG1250.066.763891
AGACGTG8300.051.1119081
CGTGTGC11600.044.9649054
ACGTGTG11600.044.365373
GCGTCGT2602.6636885E-432.456543145
GTCGATC900.00444879430.9092062
AACCGGC2250.00514308730.00427145
GTGCTCT21850.023.553247
GTGTGCT24700.022.2433665
TGTGCTC24350.021.706266
GCTCTTC26800.019.202929
TCCGATC7500.017.80370310-14
CGTTGGA10300.017.555231
ACGGGAT7750.017.0499179
TTCCGAT8800.013.90914310-14
AACGGGA10750.013.5856748
TGCTCTT38600.013.5127688
AAACGGG13400.012.4559497
CGTATAA3950.002659035812.3245571
Produced by FastQC (version 0.11.5)