FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261954
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC3800.038.7711377
TTCCGAT4550.032.3802876
CCGATCT4400.031.8896798
CTTCCGA5450.028.3203755
TCTTCCG5500.028.062924
CGTTGGA11100.017.6973361
ACGGGAT9600.016.8085179
AACGGGA12450.015.7783478
CGATCTG3357.1879465E-414.6597349
AAACGGG14500.014.5153037
GAAACGG15250.014.2614836
GGAAACG15750.014.2541815
GACGTGT3750.001685072713.0960272
GTTGGAA30350.08.5529492
CCCGAGT18903.8016879E-108.1664571
CGAGGTC9050.00207915627.7521871
CCGAGTA12952.1941732E-57.5845722
CGAGTAG13102.556561E-57.4977263
CCCATAA19555.0042217E-87.1772171
CCATAAG13102.0720987E-46.96217441
Produced by FastQC (version 0.11.5)