FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722609
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT2307.2759576E-1131.8368322
GCGTCGT4353.8758986E-514.535642145
CGTCGTG4403.237203E-414.370462145
CGCCGTA1302.474661E-413.444448135-139
GAGCGTC6355.953578E-613.2766485145
AGACGTG4608.7193574E-411.577031
ACGTGTG5854.432456E-511.3791323
CGTGTGC5904.8342674E-511.2826984
TCGCCGT1700.002349503810.501157140-144
GATCGGG2200.00551089279.580309145
ATTTCGT6950.00269713028.6203076
TCCGATC2554.7640242E-78.353621510-14
AGCGTCG5251.6875347E-68.029211145
CCCATAA14603.664638E-67.2951141
TATCCAC17801.8494939E-77.10556466
TTCCGAT2900.00134490246.88633633
CTTCCGA3902.525316E-46.82747942
TCAAGCG10050.0096099716.6236748
ATCGGAA9702.1827873E-116.5316453135-139
CCCAAAA24001.9377694E-86.37942551
Produced by FastQC (version 0.11.5)