FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937634
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG3657.0918247E-423.317108145
CGAACAT1750.00496826319.648998
CCGATGT2650.001701502219.26961145
CGTTGGA9802.910383E-1112.63149451
CGATGTA1855.803125E-410.200344140-144
CACCGAT3608.483767E-99.90126140-144
GAAACGG12351.7633283E-89.4665186
GGAAACG12903.5826815E-89.0629075
AAACGGG12351.778244E-78.9096657
AACGGGA10351.41458895E-58.6379638
GTCACCG3950.00173607468.618476145
CCCTAGT8450.00128701258.1386361
AATTCCC19100.00516397138.0205965145
TCACCGA4208.032663E-67.488348140-144
CCCTATT12651.7228394E-47.0674241
CCATAAC9850.00577676166.98187542
GGGATTC12107.9986925E-46.82031154
CCTAAAT19403.3556917E-66.3808591
AATATCC28351.1532393E-96.3070844
GGATTCC15553.1290788E-46.19164375
Produced by FastQC (version 0.11.5)