FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937634
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4158.6986984E-724.592548145
GTCGTGT4100.003628064620.743717145
GCGTCGT3252.3121302E-615.701397145
CGTCGTG3302.7105665E-515.463496145
CGTTGGA10851.8189894E-1212.6836331
AAACGGG11353.092282E-1111.5186397
ACGGGAT8509.3712515E-811.3331999
AACGGGA10259.882569E-910.7408718
GAAACGG12201.1641532E-1010.716116
CACGATC5400.00252036310.1938832
GGAAACG12201.4317266E-89.5880995
CCCTAGT8950.00225538647.68812371
GAGCGTC5401.0844269E-77.326826140-144
CCTGGTT19202.807552E-66.45081661
AGATATT39354.1873852E-46.051788145
GTAGCTG41200.06.0124126
CCCAAAT32205.511538E-105.9833661
TATCCAC18457.06566E-55.96715076
TCGGAAG6852.2748645E-75.9307384135-139
CCCAAAG60000.05.848741
Produced by FastQC (version 0.11.5)