FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320794
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT5351.392878E-411.8980979
CGTTGGA7451.9442778E-611.3923621
AAACGGG8851.3487115E-610.3893617
AACGGGA7402.5913792E-49.557788
GAAACGG8750.00137628358.0831526
CCCTGAT10551.1953108E-48.0448431
CCCTAGA9855.0507544E-47.8985111
CCTAAAG11905.7123074E-57.7265421
CCCTGAA14902.00236E-67.59490781
CCCTAAT8450.0090639417.5331151
GAGATCG8500.0095538047.48880244
GGAAACG10650.00116247377.3051965
CCAAATC15804.816684E-67.1622862
AGATATT27854.7208967E-57.155552145
CCTTAAT10950.0015606867.10505341
CCAGAAT18408.84751E-76.9190021
CATGGTA11500.0026173176.76524642
CCATGAA15051.4227694E-46.57930951
CCCAGAT15151.5508608E-46.53588151
CCCTAAA13100.00143107426.4788621
Produced by FastQC (version 0.11.5)