FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600938
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGA1050.00897620326.7712215
CCCGTAA1800.005159700319.5206811
GACGTGT3003.0672512E-416.3973712
ACGGGAT5657.309427E-814.9255479
AACGGGA8102.564775E-913.0137868
TCCGATC2250.002225589212.4932368
GGAAACG10801.09139364E-1011.7124095
AAACGGG9652.761226E-911.6517227
CGAGTAG7652.8884733E-611.0234433
CTTATCC9750.00729914510.763425145
GAAACGG10005.8003934E-810.5411676
CCGAGTA8801.362645E-610.3814532
CGTTGGA8408.8292945E-610.0392071
CAAAGCG6150.0061988689.1413921
CCCGAGT11755.8458972E-68.3731261
GTGGGAT14059.1065704E-78.0027852
CCTTACT11504.043257E-57.94406841
CCTAAAC8950.00183284917.85189341
CCCTAAA14358.310281E-56.85604431
GGATTCC11400.00255543346.7808685
Produced by FastQC (version 0.11.5)