FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600938
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG1850.00344705432.559956145
GACGTGT2553.5426838E-622.0525462
GAAACGG10150.018.6984946
ACGGGAT6201.8189894E-1218.1399979
CGTTGGA8800.017.5731221
AAACGGG10000.016.1672737
AACGGGA8700.016.1591938
GGAAACG11800.016.0838765
CGATCGG700.00248330315.961327140-144
AGACGTG3950.002453818712.4568971
TCCGATC1700.00109980612.4045569
CGTGTGC4950.001021342211.3604024
ACGTGTG5050.001207328811.1354443
CCCATAA13403.6379788E-1211.0159871
TTCGTTG8000.0058706389.411861145
GTTGGAA23350.08.1280392
GAGATTT28102.9200928E-68.038602145
TATCCAC18451.9663275E-98.0007716
TTTCGTT9400.00295893247.4779257
AATATCC22201.2369128E-97.2825554
Produced by FastQC (version 0.11.5)