FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834350
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG1101.4509272E-461.951508145
GACGTGT2551.0064997E-418.9693012
CCCGAAT4401.5969144E-615.7051041
AGACGTG5300.002082761810.4305591
AACGGGA8157.5297467E-610.1745958
GAAACGG10301.2524553E-58.7216696
AAACGGG10401.41476285E-58.6378087
CGTTGGA8550.00137858678.0821581
CCCTATT11203.1254315E-47.40383431
CCCATAA17757.1765317E-77.0075731
TATCCAC20802.7761416E-86.9766916
CCCTAAT11906.261872E-46.9683151
GGAAACG10950.0019963426.94179965
CCCAAGT27003.3287506E-106.65431071
CCCTTAA12550.00114654976.6074061
CCCGAGT11800.0043756536.44175431
GGCTAAT26057.23594E-96.36644551
CCTTAAT14900.00118896846.02907371
CCAGAAT25802.5710506E-75.89245751
CCAAATA33151.0113581E-95.8367081
Produced by FastQC (version 0.11.5)