FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966725
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT7950.086.213882
AGACGTG9850.069.583791
CGTGTGC11750.058.3319444
ACGTGTG12000.056.5278663
GTGCTCT19050.035.9790237
GTGTGCT21850.031.3684395
TGTGCTC22500.030.7762836
GCTCTTC24250.027.9725519
TCCGATC6800.020.5744810-14
TGCTCTT35750.019.5673378
CTTCCGA7750.018.41714510-14
TTCCGAT7600.018.40874510-14
CCGATCT7550.018.15630115-19
CGTTGGA9700.016.0259421
TCTTCCG9000.016.01622810-14
AAACGGG11950.012.4172097
GAAACGG11701.8189894E-1212.0786036
AACGGGA8951.0433723E-811.842438
ACGGGAT7401.8152568E-611.4583519
GGAAACG12308.367351E-1110.9149335
Produced by FastQC (version 0.11.5)