FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4320504
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG5654.641046E-420.49222145
TCCGATC4554.4532964E-513.4417047
GACGTGT7202.0896532E-611.3258792
CTCGTAT5253.8729137E-511.026767145
CGTATAA7402.903762E-611.0197741
CGTTGGA20000.010.8728451
AATCTCG14450.00170608410.6833725145
CCGATCT6309.140254E-49.7078968
TTCCGAT6309.140254E-49.7078966
CAAAGCG16605.0931703E-119.0061211
AACGGGA20800.08.8211178
AAACGGG26750.08.6373067
GAAACGG27150.08.5100546
CTTCCGA7200.0030832858.494415
GGAAACG27600.08.3713025
ACGGGAT16654.62569E-98.1627969
TATCCAC46250.07.78730736
CCCGTGG9706.5849E-47.7062681
TCTCGTA5804.7954018E-87.67473140-144
CCCATAA34350.07.31978231
Produced by FastQC (version 0.11.5)