FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4320504
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG3353.0547744E-740.004173145
GCGATCG1200.001260760231.90809145
TGGTCGC4452.8143386E-630.1155145
AGCGGCG5000.00577312919.144854145
GACGTGT6800.018.9939822
GAGCGTC11409.094947E-1016.793732145
GATCGGG7050.002163271216.293493145
CGTTGGA22200.015.9228831
CGTCGTG8101.2732926E-1114.181375145
ACGGGAT16350.014.1361929
AAACGGG25000.013.5956937
GTCGTGT9902.9467628E-1013.536766145
AACGGGA21600.013.5327498
GAAACGG27650.012.0468166
GGAAACG28650.011.151795
GCGGGAT5250.002207133510.3586232
GCGTCGT7702.7284841E-119.945379145
TAGATCT32407.14208E-89.454249145
TTCCGAT7253.1448863E-49.376349
TCCGATC5850.09.29625
Produced by FastQC (version 0.11.5)