FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395676
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA12050.022.929551
GACGTGT3553.6378442E-617.5120412
AACGGGA12150.017.0556248
AAACGGG15150.016.4139257
GAAACGG15850.015.689026
GGAAACG16650.015.350065
ACGGGAT9600.015.1102159
TCCGATC3100.00805560613.3694096
TTCCGAT4350.00575157211.1155615
AGACGTG5753.389908E-410.8117831
CGTGTGC6750.00147848349.2100364
GTTGGAA32700.08.6608132
ACGGGAA7250.0028302188.5748619
CCCTAAA23155.711627E-107.1611511
GAAATCC27850.00262905156.761929145
CCTTGAT18651.1611874E-56.2964061
CCTTAAT18851.3692254E-56.22961
CCCAAAA35051.6370905E-116.10936831
GAGATTT41457.508769E-45.78238145
TATCCAC25201.0420845E-65.7562736
Produced by FastQC (version 0.11.5)