FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572305
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT5600.046.1213842
GATCGGG2250.001896288836.780373145
AGACGTG7650.033.7620621
CGTGTGC8250.031.3066354
ACGTGTG9550.027.7567123
CGTATGC3100.00844322320.021574145
ACGTCTG9401.4970283E-917.607626145
CGTTGGA12400.016.4439561
ACGGGAT9350.014.5386879
AAACGGG14400.013.2160697
TCCGATC4650.013.1551669
AACGGGA12500.013.0499258
GTGTGCT20800.012.7440675
GTGCTCT22750.012.2492437
GAAACGG16000.011.8944636
AAGCGGC4800.001046383311.3280593
GGAAACG15900.011.1143235
CCGATCT4850.010.93099315-19
TGTGCTC25300.010.7459856
GCTCTTC26750.010.417589
Produced by FastQC (version 0.11.5)