FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572305
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGG300.008593682135.96849145
GTCGCCG1758.430503E-658.27221145
GACGTGT6800.055.0022282
CGACGTG1101.6489685E-743.274481
AGACGTG8000.039.951621
GGATCGG2659.78839E-538.481647145
CGTGTGC10400.035.9629944
ACGTGTG11350.034.151163
GCTTCGT3605.9124257E-428.326769145
GAGCTCG3100.00975558126.316483145
AGACCGG4759.84654E-525.762451145
ATTCGCC1950.00989816517.4366026
GTGCTCT24900.016.1131027
CGTTGGA11800.015.5599521
TCCGATC5600.015.54348510-14
GTCGTGT5308.479947E-815.39266145
GTGTGCT23900.015.3646395
GCTCTTC25250.015.3511159
TGTGCTC26900.014.9151016
CGTCGTG5553.6379788E-1214.699296145
Produced by FastQC (version 0.11.5)