FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797551
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT2900.031.6610222
AGACGTG5553.086825E-916.5435981
CGTGTGC6008.6638465E-915.3028294
ACGTGTG5901.16806405E-714.3651073
CGTTGGA8254.3095497E-811.9854321
CACGATC4959.814777E-411.4146982
ACGGGAT5702.5440665E-411.1518599
AACGGGA8356.501359E-710.9960448
GCACGAT4650.0080020710.6322381
CCCGAAT5500.002363101810.2732281
GAAACGG10451.02696504E-710.1380546
TTCCGAT2806.9267044E-610.0897778
AACCCCG11550.00303817829.965611145
CAAAGCG6050.00519786549.3392971
TCCGATC2451.04530045E-79.22493910-14
GGAAACG11303.2285006E-68.7504265
AAACGGG10651.4492038E-58.6213127
GATCGGA2754.7690552E-47.1370373135-139
GGATATT27002.1978615E-46.9274845145
CCCAATT12357.4531784E-46.8626821
Produced by FastQC (version 0.11.5)