FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797551
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3700.040.093852
CGACGTG850.00311482133.2430761
GATCGGG2150.00867741926.956377145
AGACGTG5350.022.4468441
CGTGTGC7300.022.2569224
ACGTGTG7950.021.325753
CGTTGGA10000.016.2475551
ACGGGAT6751.5279511E-1015.698129
AACGGGT3154.2746126E-415.698128
GGAAACG11350.013.6926345
TCCGATC3100.013.21680510-14
ACGGGTT3250.00949014113.0415159
GAAACGG11950.013.0051376
AAACGGG11700.012.6792527
AACGGGA9201.5741534E-811.5176438
TTCCGAT3750.010.54913710-14
CCGATCT3400.09.97292315-19
GTGTGCT17200.09.8569595
GTGCTCT18401.8189894E-129.2141147
TGTGCTC19054.3655746E-118.52896
Produced by FastQC (version 0.11.5)