FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576637
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3700.032.382232
AGACGTG5150.023.2649041
TCCGATC3105.833499E-922.7351537
ACGTGTG5600.022.6539553
CCGATCT3851.4568286E-820.136858
CGTGTGC6453.6379788E-1217.4831544
ACGGGAT7151.8189894E-1216.7572369
TTCCGAT4353.220739E-716.2020656
CGTTGGA8950.015.7494911
CTTCCGA4501.6429512E-615.6619935
AACGGGA9300.015.1567688
AAACGGG11100.013.9688057
GGAAACG11950.012.3854265
GAAACGG12100.012.2318886
TCTTCCG5902.3263528E-611.9455884
CGTGATC8901.694005E-59.5027821
GTGCTCT14652.1082087E-99.1406177
CCAACGA6200.00646891729.0940618
CCCAAGT24100.08.7733161
CCAAGTA21609.094947E-128.1572892
Produced by FastQC (version 0.11.5)