FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069113
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3101.2660166E-924.8836352
TCCGATC3201.8280844E-924.1060227
CCGATCT4254.7972208E-818.1504178
TTCCGAT4055.761831E-717.3152126
CACACGT8501.0960062E-515.591954145
CGTTGGA9850.014.9508511
AACGGGT2850.004173834314.7634978
CTTCCGA4904.194455E-614.3115535
AGACGTG5601.1008688E-613.7748691
AAACGGG14050.012.4780437
GAAACGG13550.011.9034096
AACGGGA10658.913048E-1111.8523858
TCTTCCG6154.3408727E-511.4026994
CCGAGTA11402.928573E-1011.0726232
GGAAACG12902.0008883E-1110.8723425
ACGGGAT7302.1218502E-510.5670239
CGTGTGC7302.1218502E-510.5670234
CCCGAGT16850.010.4045411
CGAGTAG10651.5034675E-79.87698653
TCAAGCG12601.7789716E-89.4615278
Produced by FastQC (version 0.11.5)