FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005618
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT5000.055.237762
AGCGTCG1951.5178179E-435.718227145
ACGTGTG8100.034.097383
CGTGTGC7750.033.809714
AGACGTG7900.033.1677551
GTGCTCT18500.016.4603167
AACGGGT4101.1287166E-515.5453358
GTGTGCT19250.014.3474695
TCCGATC5050.014.02329410-14
TGTGCTC21450.013.866396
ACGGGTT3750.001570188313.2192939
CGTTGGA10403.6379788E-1212.9378371
GCTCTTC21900.012.6113619
CGTATAA4250.004015506211.6640811
CCGATCT5900.010.802698
TTCCGAT6100.010.44850356
GAAACGG14251.1277734E-109.9393186
GGAAACG13605.0386006E-109.893645
CTTCCGA6500.09.8055195
AAACGGG13756.148184E-109.7857097
Produced by FastQC (version 0.11.5)