FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419181
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3850.052.369312
GTGATCG1652.955036E-547.14121145
ACGTGTG5300.038.041863
AGACGTG5350.037.6863251
CGTGTGC5400.037.337384
ATGATCG2251.838383E-434.57022145
GGCGAAG2207.8288815E-418.9613021
CACCGAT2550.002709960618.301882145
GTGTGCT11950.017.4539175
CGTTGGA8100.017.1666091
TGTGCTC12850.016.2314646
GTGCTCT12900.016.1685527
TCCGATC2950.016.02604910-14
TATACCC7356.624167E-414.815809145
GCTCTTC16150.013.3453139
CCGATCT3200.013.0358948
AAACGGG9251.2369128E-1012.7775267
GAAACGG10104.0017767E-1112.39055256
GGAAACG10154.3655746E-1112.3295155
AACGGGA7602.3562461E-711.8923958
Produced by FastQC (version 0.11.5)