FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249472
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT2251.1754273E-625.0352842
AGACGTG3901.3456958E-414.4434321
TCCGATC2300.00274742312.2455195
ACGTGTG4950.001006681611.3796743
CGTGTGC4950.001006681611.3796744
CGTTGGA5752.8368982E-411.02096751
CCTTATT13002.7645001E-89.2076881
GAAACGG7854.895406E-48.9696476
CCCAAAG37550.08.6256721
AAAGTGC30550.07.60585074
GCCTAAA8450.0094325027.49947451
CCAAAGT34150.07.42261362
CCCAAAT22151.1368684E-97.31137661
CCTCAAT10750.0013467.20492121
AAGTGCT32400.06.95424565
CAAAGTG35700.06.90311153
CCTAGAA13302.3839298E-46.882351
CCAAATA23202.1969754E-86.67697531
CCTGGAT12750.00110792046.62698651
CCAAGTA15954.4862434E-56.62179332
Produced by FastQC (version 0.11.5)