FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067537
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3350.073.814042
AGACGTG4000.058.3848531
CGTGTGC5350.047.5038954
ACGTGTG5400.045.792043
CGTCGTG2050.002355616835.185814145
GTGCTCT11150.023.4093867
GTGTGCT10900.023.3161325
TGTGCTC11250.021.980186
GCTCTTC13350.020.0661779
TCCGATC3350.017.63335410-14
TGCTCTT17900.016.1167538
CCGATCT3750.013.55444415-19
GGAAACG6354.012436E-712.9804215
TTCCGAT4450.012.96583710-14
CGTTGGA4455.068495E-412.3484161
CGTGAAC4000.003204651212.02041151
CTTCCGA4550.011.77509710-14
CGATCTA1051.5360948E-411.77509715-19
CTACTCG5653.0328377E-410.9414625
TCTTCCG4800.010.8756110-14
Produced by FastQC (version 0.11.5)