FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873372
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT1700.003846593420.5356662
TGCCTCG3600.001283367313.5763574
GAAACGG5400.00223032910.343896
TTCCGTT5650.00324622549.8861962
CCTAGGA7754.6813668E-49.0091941
CCCAGTT9750.00450943557.16115471
CCCAAGT12701.4976594E-47.14705851
GTTGGAA12400.00657446366.19382142
CTGAGGT17350.0063870375.2315642
Produced by FastQC (version 0.11.5)