FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570053
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAA1102.802424E-432.1787722
CCCGATA900.00407773931.4636861
TCCGATC1805.659329E-627.5307257
TTCCGAT2303.777796E-521.5457866
CTTCCGA3001.3584882E-518.8782125
CCGATCT2701.296006E-418.3538178
CGTTGGA9000.018.091621
ACGGGAT6453.6379788E-1217.5611279
AAACGGG11700.016.3369147
ACGGGTT2850.003918806614.90385159
AACGGGA9300.014.4631468
GAAACGG11850.014.3378826
CAGAACG3509.3507476E-414.1586582
GGAAACG11600.014.0366015
TCTTCCG3600.001156915213.7653634
CTATTCG3250.00935597913.06953056
GTTGGAA23100.08.2745412
CCTTGAC11202.649346E-58.2170781
CCCAAGT20903.45608E-118.1293741
CCCAAAG44650.07.45192581
Produced by FastQC (version 0.11.5)