FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557293
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45460.2919168069207272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG2302.0522217E-433.92932145
TCCGATC2750.032.8546077
CCGATCT3051.8189894E-1229.6230058
TTCCGAT3353.6379788E-1226.97026
CTTCCGA3852.910383E-1123.4675775
GCGGCGG4050.005559298719.268503145
TCTTCCG5151.4133548E-917.5437224
CGTTGGA7552.1731466E-711.966911
TAGACGA900.00704053310.81789250-54
AACGGGA8005.6327226E-610.4250198
ATCGGGA1751.4993903E-410.092617135-139
CCGAGTA7754.523915E-59.8645342
CCCTAAA14803.2923708E-109.3919091
CGAGTAG7453.3060036E-49.32887653
AAACGGG9655.1798765E-58.6425037
CCCAAAG49950.07.9309461
CCCAAAT25601.8189894E-127.6015771
CCCAAGT19708.740244E-97.40864321
GAAACGG10500.00120456717.2809666
GTAGCTG32800.07.2042826
Produced by FastQC (version 0.11.5)