FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1184741
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT2700.002693935815.766211
CCCTAGT5551.3448189E-512.7834131
CCCTAAT8054.7402173E-610.576091
CCGAGTA7602.9070228E-510.2687812
AAACGGG7352.3456612E-49.6527817
GAAACGG7553.0717353E-49.3970786
CCCGAGT9352.8029002E-59.1056181
CGAGTAG7854.537658E-49.0379543
CCATAGA7904.8348226E-48.9807521
CCTTAAA14159.6441E-88.5237811
CCTATTC8050.0057098247.93206742
CCAAGAA17453.4803998E-77.31841231
CCCAACT11150.00182833956.9993481
CCTGAGT20651.10650944E-76.8714711
CCAGGAT17603.0991323E-66.85292631
CCCTGAA12900.00116059046.59980871
CCCAAAG39400.06.4825531
CCAGAAA21501.609953E-66.2698181
GAGTAGC19459.7209115E-55.8363344
CCTGAAG14700.0050225985.79166841
Produced by FastQC (version 0.11.5)