FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112836
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG1959.094947E-1173.19714145
GACGTGT3455.7379122E-515.9626712
GCGTCGT4551.368652E-515.6851015145
AGCGCTT8200.00941995413.054977145
CGTCGTG4102.1762187E-413.054977145
TCCGATC2200.00218312412.5161864
GTCGTGT6052.068605E-511.796234145
ACGGGAT6851.2882603E-511.05444059
AGCGTCG5350.011.038123140-144
AACGGGA8409.775868E-710.6536588
ATCGGAA7250.010.047871135-139
GAAACGG12051.2016426E-79.14045056
GATCGGA6800.09.021308135-139
AAACGGG10501.6750939E-58.5229267
GAGCGTC6352.3646862E-118.2665205140-144
CGTTGGA10151.0454722E-48.1386031
AGAGCGT6901.8189894E-117.924578140-144
TGGTCGC2500.00610746357.8738613140-144
GGAAACG12059.3464245E-57.42661575
AAGAGCG10350.07.3962727140-144
Produced by FastQC (version 0.11.5)