FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893108
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCG5050.048.187675145
CTGATCG3602.6193447E-1046.797646145
GACGTGT6250.038.2578242
GTGATCG4258.2828556E-526.426908145
AGACGTG10150.022.8846951
ACGTGTG11950.020.0093233
CGTGTGC11750.019.1870564
CGATCTA2350.001371507817.442789
TCCGATC5850.015.1816777
GACCGGA3850.014.770668140-144
CGTTGGA13950.013.2227511
CCGATCT7300.013.10199458
AGTTCGG3601.4551915E-1112.00527140-144
TTCCGAT7550.010.85841756
AAACGGG16100.010.1839827
AACGGGA13508.458301E-109.6150628
TATGCTC15750.0044818319.508093145
CTTCCGA8750.09.3692635
AGACCGG6800.09.366342140-144
GTGTGCT26500.09.2808745
Produced by FastQC (version 0.11.5)