FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861851
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT2700.036.4569242
TCCGATC2851.2914825E-1017.269079
AGACGTG5654.152753E-916.1774731
CGTGTGC7252.910383E-1115.5166424
CGTTGGA9751.8189894E-1213.7013931
ACGTGTG6855.2536052E-813.3434633
TTCCGAT3401.5614452E-512.4076095
ACGGGAT6253.667219E-612.3745229
CCGATCT3650.001820287211.5577737
GGAAACG12307.2759576E-1211.4324855
AAACGGG11152.2919266E-910.7198777
GAAACGG12107.912604E-1010.4593076
AACGGGA8851.4564866E-610.327998
GATCGGA3306.479313E-78.186102135-139
CCCTAAT10751.5934068E-47.8485341
GTTGGAA25759.094947E-127.37228782
CCAAAGT52800.07.19077352
CCCAATG13754.71104E-57.15881441
GTGCTCT16951.9815852E-67.0517197
CCCAAAG57000.06.9076281
Produced by FastQC (version 0.11.5)