FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879179
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3452.72923E-618.0470432
ACGGGAT5902.7284841E-1117.5882199
TCCGATC2501.654126E-516.603286
AGTTCCG2500.001907651516.603285
AACGGGA8450.015.5553418
AAACGGG10700.014.2239937
CGTTGGA10000.013.8360661
GAAACGG12050.013.7786566
CCGATCT3100.001884348613.3897427
TTCCGAT3150.00886346313.1772065
GGAAACG12150.012.5264815
CTTCCGA4000.003039272712.1065587
AGACGTG4900.001072255511.2947481
CGTGTGC5753.341867E-410.8282264
CGTTTCC11350.00792920210.6440525145
CCCATAA15054.9294613E-109.19341
ACGTGTG6850.0016669169.0893873
TATCCAC19701.1768861E-97.72572236
CCACATA15606.893533E-77.5388821
CCCAAGT25309.094947E-127.3828811
Produced by FastQC (version 0.11.5)